phrs surrogate reporter plasmid (PNA Bio Inc)
90
Structured Review
PNA Bio Inc
phrs surrogate reporter plasmid
Phrs Surrogate Reporter Plasmid, supplied by PNA Bio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrs+surrogate+reporter+plasmid/phrs+surrogate+reporter+plasmid/10__1681_slash_asn__0000000000000084-35-10-14
Average 90 stars, based on 1 article reviews
Phrs Surrogate Reporter Plasmid, supplied by PNA Bio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phrs+surrogate+reporter+plasmid/phrs+surrogate+reporter+plasmid/10__1681_slash_asn__0000000000000084-35-10-14
Average 90 stars, based on 1 article reviews
phrs surrogate reporter plasmid - by Bioz Stars,
2026-09
90/100 stars
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Related Articles
Sequencing:Article Title: Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model Article Snippet: The annealed oligo duplexes were cloned into the pX330 vector in a single-step digestion ligation reaction containing 100 ng pX330, oligo duplex (1:150 dilution), 1 ml FastDigest BbsI (Thermo Fisher Scientific), 0.5 ml T7 DNA ligase (New England Biolabs), and ddH2O in a total volume of 20 ml with thermocycler settings at 37°C for 5 minutes and 23°C for 5 minutes (six cycles total). .. The same sgRNA sequence with added PAM sequence for nuclease binding/activation (Supplemental Table 1) was inserted into the Article Title: Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model Article Snippet: The annealed oligo duplexes were cloned into the pX330 vector in a single-step digestion ligation reaction containing 100 ng pX330, oligo duplex (1:150 dilution), 1 μ l FastDigest BbsI (Thermo Fisher Scientific), 0.5 μ l T7 DNA ligase (New England Biolabs), and ddH 2 O in a total volume of 20 μ l with thermocycler settings at 37°C for 5 minutes and 23°C for 5 minutes (six cycles total). .. The same sgRNA sequence with added PAM sequence for nuclease binding/activation ( Supplemental Table 1 ) was inserted into the Binding Assay:Article Title: Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model Article Snippet: The annealed oligo duplexes were cloned into the pX330 vector in a single-step digestion ligation reaction containing 100 ng pX330, oligo duplex (1:150 dilution), 1 ml FastDigest BbsI (Thermo Fisher Scientific), 0.5 ml T7 DNA ligase (New England Biolabs), and ddH2O in a total volume of 20 ml with thermocycler settings at 37°C for 5 minutes and 23°C for 5 minutes (six cycles total). .. The same sgRNA sequence with added PAM sequence for nuclease binding/activation (Supplemental Table 1) was inserted into the Article Title: Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model Article Snippet: The annealed oligo duplexes were cloned into the pX330 vector in a single-step digestion ligation reaction containing 100 ng pX330, oligo duplex (1:150 dilution), 1 μ l FastDigest BbsI (Thermo Fisher Scientific), 0.5 μ l T7 DNA ligase (New England Biolabs), and ddH 2 O in a total volume of 20 μ l with thermocycler settings at 37°C for 5 minutes and 23°C for 5 minutes (six cycles total). .. The same sgRNA sequence with added PAM sequence for nuclease binding/activation ( Supplemental Table 1 ) was inserted into the Plasmid Preparation:Article Title: Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model Article Snippet: The annealed oligo duplexes were cloned into the pX330 vector in a single-step digestion ligation reaction containing 100 ng pX330, oligo duplex (1:150 dilution), 1 ml FastDigest BbsI (Thermo Fisher Scientific), 0.5 ml T7 DNA ligase (New England Biolabs), and ddH2O in a total volume of 20 ml with thermocycler settings at 37°C for 5 minutes and 23°C for 5 minutes (six cycles total). .. The same sgRNA sequence with added PAM sequence for nuclease binding/activation (Supplemental Table 1) was inserted into the Article Title: Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model Article Snippet: The annealed oligo duplexes were cloned into the pX330 vector in a single-step digestion ligation reaction containing 100 ng pX330, oligo duplex (1:150 dilution), 1 μ l FastDigest BbsI (Thermo Fisher Scientific), 0.5 μ l T7 DNA ligase (New England Biolabs), and ddH 2 O in a total volume of 20 μ l with thermocycler settings at 37°C for 5 minutes and 23°C for 5 minutes (six cycles total). .. The same sgRNA sequence with added PAM sequence for nuclease binding/activation ( Supplemental Table 1 ) was inserted into the Article Title: Impaired Endosome Maturation Mediates Tubular Proteinuria in Dent Disease Cell Culture and Mouse Models Article Snippet: Background Loss of function of the 2Cl/H antiporter ClC-5 in Dent disease impairs the uptake of filtered proteins by the kidney proximal tubule, resulting in tubular proteinuria.. Reduced posttranslational stability of megalin and cubilin, the receptors that bind to and recover filtered proteins, is believed to underlie the tubular defect.. How loss of ClC-5 leads to reduced receptor expression remains unknown. CRISPR:Article Title: Impaired Endosome Maturation Mediates Tubular Proteinuria in Dent Disease Cell Culture and Mouse Models Article Snippet: Background Loss of function of the 2Cl/H antiporter ClC-5 in Dent disease impairs the uptake of filtered proteins by the kidney proximal tubule, resulting in tubular proteinuria.. Reduced posttranslational stability of megalin and cubilin, the receptors that bind to and recover filtered proteins, is believed to underlie the tubular defect.. How loss of ClC-5 leads to reduced receptor expression remains unknown. Generated:Article Title: Impaired Endosome Maturation Mediates Tubular Proteinuria in Dent Disease Cell Culture and Mouse Models Article Snippet: Background Loss of function of the 2Cl/H antiporter ClC-5 in Dent disease impairs the uptake of filtered proteins by the kidney proximal tubule, resulting in tubular proteinuria.. Reduced posttranslational stability of megalin and cubilin, the receptors that bind to and recover filtered proteins, is believed to underlie the tubular defect.. How loss of ClC-5 leads to reduced receptor expression remains unknown. Recombinant:Article Title: Impaired Endosome Maturation Mediates Tubular Proteinuria in Dent Disease Cell Culture and Mouse Models Article Snippet: Background Loss of function of the 2Cl/H antiporter ClC-5 in Dent disease impairs the uptake of filtered proteins by the kidney proximal tubule, resulting in tubular proteinuria.. Reduced posttranslational stability of megalin and cubilin, the receptors that bind to and recover filtered proteins, is believed to underlie the tubular defect.. How loss of ClC-5 leads to reduced receptor expression remains unknown. |